All the three brands of insulin for both the formulations did not differ considerably in their insulin content during various sampling time and temperatures (pharmaceutically equivalent). There was no difference in concentration of insulin in vials stored at 25 and 26C compared to those stored at 5°C on all five sampling days. There was a 14 and 18 per cent decrease in the concentration of regular insulin in vials stored at 32 and 37°C on day 28 for all the three brands. There was a 11 and 14 per cent decrease in the concentration of regular insulin in biphasic insulin formulation in vials stored at 32 and 37°C on day 28.